全文获取类型
收费全文 | 892篇 |
免费 | 46篇 |
出版年
2023年 | 1篇 |
2022年 | 5篇 |
2021年 | 14篇 |
2020年 | 8篇 |
2019年 | 5篇 |
2018年 | 10篇 |
2017年 | 11篇 |
2016年 | 14篇 |
2015年 | 35篇 |
2014年 | 27篇 |
2013年 | 64篇 |
2012年 | 63篇 |
2011年 | 49篇 |
2010年 | 40篇 |
2009年 | 32篇 |
2008年 | 55篇 |
2007年 | 57篇 |
2006年 | 71篇 |
2005年 | 38篇 |
2004年 | 46篇 |
2003年 | 42篇 |
2002年 | 41篇 |
2001年 | 11篇 |
2000年 | 11篇 |
1999年 | 15篇 |
1998年 | 8篇 |
1997年 | 6篇 |
1996年 | 5篇 |
1995年 | 7篇 |
1994年 | 12篇 |
1993年 | 12篇 |
1992年 | 13篇 |
1991年 | 18篇 |
1990年 | 10篇 |
1989年 | 8篇 |
1988年 | 15篇 |
1987年 | 8篇 |
1986年 | 5篇 |
1985年 | 6篇 |
1984年 | 10篇 |
1983年 | 5篇 |
1982年 | 1篇 |
1981年 | 5篇 |
1980年 | 4篇 |
1978年 | 2篇 |
1975年 | 1篇 |
1974年 | 4篇 |
1973年 | 2篇 |
1972年 | 4篇 |
1971年 | 2篇 |
排序方式: 共有938条查询结果,搜索用时 46 毫秒
1.
Walter Soares Leal Yasumasa Kuwahara Takahisa Suzuki Kazuyoshi Kurosa 《Bioscience, biotechnology, and biochemistry》2013,77(10):2703-2709
The stored-product mite Suidasia medanensis has been found in human-provided habitats in Japan. The mite uses neral as the alarm pheromone, and also secretes (Z,E)- and (£,£)-farnesal, which were isolated from an astigmatid mite for the first time. On the other hand, S. medanensis is the only mite out of fifteen species investigated so far that does not secrete volatile hydrocarbons along with the alarm pheromone, though 9-nonadecene and 6,9-nonadecadiene were found in a hexane extract of the mite. GC-MS, JH-NMR and 13C-NMR of the four isomers of synthetic farnesal, which were isolated by LC and preparative GLC, cleared up some confusion in the literature concerning the stereochemistry of this sesquiterpenoid. 相似文献
2.
T Yamazaki Y Hamano H Tashiro K Itoh H Nakano S Miyatake T Saito 《The Journal of biological chemistry》1999,274(26):18173-18180
Antigen recognition through T cell receptor (TCR)-CD3 complex transduces signals into T cells, which regulate activation, function, and differentiation of T cells. The TCR-CD3 complex is composed of two signaling modules represented by CD3zeta and CD3epsilon. Signaling through CD3zeta has been extensively analyzed, but that via CD3epsilon, which is also crucial in immature thymocyte development, is still not clearly understood. We isolated cDNA encoding a novel CD3epsilon-binding protein CAST. CAST specifically interacts in vivo and in vitro with CD3epsilon but not with CD3zeta or FcRgamma via a unique membrane-proximal region of CD3epsilon. CAST is composed of 512 amino acids including a single tyrosine and undergoes tyrosine phosphorylation upon TCR stimulation. Overexpression of two dominant-negative types of CAST, a minimum CD3epsilon-binding domain and a tyrosine-mutant, strongly suppressed NFAT activation and interleukin-2 production. These results demonstrate that CAST serves as a component of preformed TCR complex and transduces activation signals upon TCR stimulation and represents a new signaling pathway via the CD3epsilon-containing TCR signaling module. 相似文献
3.
4.
Kazutaka Miyatake Yoshihisa Nakano Shozaburo Kitaoka 《Bioscience, biotechnology, and biochemistry》2013,77(5):1205-1207
When yeast protoplasts that were producing repressible acid phosphatase (r-APase) were treated with tunicamycin (TM), three specific proteins of 59k, 57k, and 55k daltons were accumulated in the membrane fraction in addition to the usual membrane proteins and these proteins were not detected in the secreted fraction. These proteins were immunoprecipitated with anti r-APase antiserum. Their molecular sizes were almost the same as those endo-H treated r-APase. Therefore these proteins were considered to be nonglycosylated forms of r-APase proteins. These results proved that nonglycosylated forms of r-APase produced by TM-treatment were not secreted by yeast protoplasts. 相似文献
5.
6.
7.
Journal of Ethology - Males of the Japanese scorpionfly, Panorpa japonica, often perform male–male competitions for food to offer to females as a nuptial gift. Previous studies have suggested... 相似文献
8.
Journal of Ethology - Circadian rhythm is an important factor for long-distance movement in insect species. Previous studies have shown the relationship between movement and circadian rhythm,... 相似文献
9.
Masayoshi Shinjoh Norio Sugaya Yoshio Yamaguchi Yuka Tomidokoro Shinichiro Sekiguchi Keiko Mitamura Motoko Fujino Hiroyuki Shiro Osamu Komiyama Nobuhiko Taguchi Yuji Nakata Naoko Yoshida Atsushi Narabayashi Michiko Myokai Masanori Sato Munehiro Furuichi Hiroaki Baba Hisayo Fujita Akihiro Sato Ichiro Ookawara Kenichiro Tsunematsu Makoto Yoshida Mio Kono Fumie Tanaka Chiharu Kawakami Takahisa Kimiya Takao Takahashi Satoshi Iwata Keio Pediatric Influenza Research Group 《PloS one》2015,10(8)
We assessed vaccine effectiveness (VE) against medically attended, laboratory-confirmed influenza in children 6 months to 15 years of age in 22 hospitals in Japan during the 2013–14 season. Our study was conducted according to a test-negative case-control design based on influenza rapid diagnostic test (IRDT) results. Outpatients who came to our clinics with a fever of 38°C or over and had undergone an IRDT were enrolled in this study. Patients with positive IRDT results were recorded as cases, and patients with negative results were recorded as controls. Between November 2013 and March 2014, a total of 4727 pediatric patients (6 months to 15 years of age) were enrolled: 876 were positive for influenza A, 66 for A(H1N1)pdm09 and in the other 810 the subtype was unknown; 1405 were positive for influenza B; and 2445 were negative for influenza. Overall VE was 46% (95% confidence interval [CI], 39–52). Adjusted VE against influenza A, influenza A(H1N1)pdm09, and influenza B was 63% (95% CI, 56–69), 77% (95% CI, 59–87), and 26% (95% CI, 14–36), respectively. Influenza vaccine was not effective against either influenza A or influenza B in infants 6 to 11 months of age. Two doses of influenza vaccine provided better protection against influenza A infection than a single dose did. VE against hospitalization influenza A infection was 76%. Influenza vaccine was effective against influenza A, especially against influenza A(H1N1)pdm09, but was much less effective against influenza B. 相似文献